Spore-spheroplast transformation of Bacillus megaterium.
نویسندگان
چکیده
منابع مشابه
Biolistic transformation of a procaryote, Bacillus megaterium.
We present a simple and rapid method for introducing exogenous DNA into a bacterium, Bacillus megaterium, utilizing the recently developed biolistic process. A suspension of B. megaterium was spread onto the surface of nonselective medium. Plasmid pUB110 DNA, which contains a gene that confers kanamycin resistance, was precipitated onto tungsten particles. Using a biolistic propulsion system, t...
متن کاملSpore germination of Bacillus megaterium QM B1551 mutants.
Auxotrophic or antibiotic-resistant mutants of Bacillus megaterium QM B1551 are capable of initiation of germination similar to the parental (QM B1551) prototrophic strain.
متن کاملBacillus megaterium spore germination is influenced by inoculum size.
AIMS The effect of spore density on the germination (time-to-germination, percent germination) of Bacillus megaterium spores on tryptic soy agar was determined using direct microscopic observation. METHODS AND RESULTS Inoculum size varied from approximately 10(3) to 10(8) cfu ml(-1) in a medium where pH=7 and the sodium chloride concentration was 0.5% w/v. Inoculum size was measured by global...
متن کاملCloning of a new low-molecular-weight spore-specific protein gene from Bacillus megaterium.
Three EcoRI fragments of Bacillus megaterium DNA hybridized only under nonrestrictive conditions on Southern blots to a probe containing the previously cloned gene for protein C, a small, acid-soluble spore protein (SASP) from B. megaterium. All three fragments were cloned in Escherichia coli cells in plasmid pBR325, and after being transferred to an E. coli expression vector, one of the fragme...
متن کاملCharacterization of the germination of Bacillus megaterium spores lacking enzymes that degrade the spore cortex.
AIMS To determine roles of cortex lytic enzymes (CLEs) in Bacillus megaterium spore germination. METHODS AND RESULTS Genes for B. megaterium CLEs CwlJ and SleB were inactivated and effects of loss of one or both on germination were assessed. Loss of CwlJ or SleB did not prevent completion of germination with agents that activate the spore's germinant receptors, but loss of CwlJ slowed the rel...
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ژورنال
عنوان ژورنال: Nippon Saikingaku Zasshi
سال: 1990
ISSN: 1882-4110,0021-4930
DOI: 10.3412/jsb.45.841